Vitamin B3 (Niacin): Reference and Dietary Sources

We identified Vitamin B3 (Niacin) dietary sources, such as poultry, beef, and fish, provided reference values, and described testing methods, benefits, and toxicity. Nutri-IQ recommends to Wellness Practitioners to check nutritional status with our Nutritional Balance Assessment Tool in order to holistically recognize and close nutritional gaps.


In this article, we describe:

  • the major purposes of this specific nutrient in the human body, 
  • its experimentally confirmed health uses, 
  • conventional ways to estimate nutrient status,
  • nutrient’s toxicities and deficiencies,
  • experimentally confirmed and approved levels of the nutrient intake for different demographics,
  • dietary sources of the nutrient.


Niacin (also known as vitamin B3) is one of the water-soluble B vitamins. Niacin is the generic name for nicotinic acid (pyridine-3-carboxylic acid), nicotinamide (niacinamide or pyridine-3-carboxamide), and related derivatives, such as nicotinamide riboside. Niacin is naturally present in many foods, added to some food products, and available as a dietary supplement.

B Complex vitamins are all water soluble and are not stored very well in the body. Thus, they are needed daily through diet or supplement to support their many functions. Deficiencies of one or more of the B vitamins may occur fairly easily, especially during times of fasting or dieting for weight loss or with diets that include substantial amounts of refined and processed food, sugar, or alcohol.

All tissues in the body convert absorbed niacin into its main metabolically active form, the coenzyme nicotinamide adenine dinucleotide (NAD). More than 400 enzymes require NAD to catalyze reactions in the body, which is more than for any other vitamin-derived coenzyme. NAD is also converted into another active form, the coenzyme nicotinamide adenine dinucleotide phosphate (NADP), in all tissues except skeletal muscle.

B complex vitamins are fairly easily digested from food or supplements and then absorbed into the blood, mainly from the small intestine. When the amount of Bs taken exceeds the body’s needs, the excess is easily excreted in the urine, giving it a dark yellow color. 

The most sensitive and reliable measure of niacin status is the urinary excretion of its two major methylated metabolites, N1-methyl-nicotinamide and N1-methyl-2-pyridone-5-carboxamide. Excretion rates in adults of more than 17.5 micromol/day of these two metabolites reflect adequate niacin status, while excretion rates between 5.8 and 17.5 micromol/day reflect low niacin status. An adult has deficient niacin status when urinary-excretion rates are less than 5.8 micromol/day. Indicators of inadequacy such as this and other biochemical signs (e.g., a 2-pyridone oxidation product of N1-methyl-nicotinamide below detection limits in plasma or low erythrocyte NAD concentrations) occur well before overt clinical signs of deficiency. Another measure of niacin status takes into account the fact that NAD levels decline as niacin status deteriorates, whereas NADP levels remain relatively constant. A “niacin number” (NAD/[NAD + NADP] concentrations in whole blood x 100) below 130 suggests niacin deficiency. A “niacin index” (the ratio of erythrocyte NAD to NADP concentrations) below 1 suggests that an individual is at risk of developing niacin deficiency. No functional biochemical tests that reflect total body stores of niacin are available.

Severe niacin deficiency leads to pellagra, a disease characterized by a pigmented rash or brown discoloration on skin exposed to sunlight; the skin also develops a roughened, sunburned-like appearance. As pellagra progresses, anorexia develops, and the affected individual eventually dies. Niacin inadequacy usually arises from insufficient intakes of foods containing niacin and tryptophan. It can also be caused by factors that reduce the conversion of tryptophan to niacin, such as low intakes of other nutrients. The following groups are among those most likely to have inadequate niacin status: malnourished individuals; people with Hartnup disease; and people with carcinoid syndrome. Despite numerous claims, experts do not agree on the value of nicotinic acid to treat cardiovascular disease, especially given its side effects, safety concerns, and poor patient compliance.

No adverse effects have been reported from the consumption of naturally occurring niacin in foods. However, high intakes of both nicotinic acid and nicotinamide taken as a dietary supplement or medication can cause adverse effects, including hepatotoxicity.

Assessment of nutritional balance brings cost-effective immediate answers when your client:

  • Feels stressed, tired, depressed
  • Takes prescription meds but cannot alleviate symptoms
  • Wants to achieve optimal wellness, prevent chronic disease, and manage aging process

Nutri-IQis a unique tool that helps Wellness Professionals to easy and conveniently identify clients’ nutritional gaps as possible causes for clients’ complaints.

Recommended Dietary Allowances (RDAs) for Niacin (Vitamin B3) 

Birth to 6 months*2 mg2 mg  
7–12 months*4 mg NE4 mg NE  
1–3 years6 mg NE6 mg NE  
4–8 years8 mg NE8 mg NE  
9–13 years12 mg NE12 mg NE  
14–18 years16 mg NE14 mg NE18 mg NE17 mg NE
19+ years16 mg NE14 mg NE18 mg NE17 mg NE
  • * Intake at this level is assumed to ensure nutritional adequacy (Adequate Intake or AI), established when evidence is insufficient to develop an RDA.  The AI for infants from birth to 6 months is for niacin alone, as young infants use almost all the protein they consume for growth and development; it is equivalent to the mean intake of niacin in healthy, breastfed infants. For infants aged 7-12 months, the AI for niacin is in mg NE and is based on amounts consumed from breast milk and solid foods.
  • The current RDAs for niacin as mg of niacin equivalents (NE). 1 NE is defined as 1 mg niacin or 60 mg of the amino acid tryptophan (which the body can convert to niacin). 
  • Niacin RDAs for adults are based on niacin metabolite excretion data. For children and adolescents, niacin RDAs are extrapolated from adult values on the basis of body weight.

Food Sources of Niacin

Niacin is present in a wide variety of foods. Many animal-based foods—including poultry, beef, and fish—provide about 5-10 mg niacin per serving, primarily in the highly bioavailable forms of NAD and NADP. Plant-based foods, such as nuts, legumes, and grains, provide about 2-5 mg niacin per serving, mainly as nicotinic acid. In some grain products, however, naturally present niacin is largely bound to polysaccharides and glycopeptides that make it only about 30% bioavailable. Many breads, cereals, and infant formulas in the United States and many other countries contain added niacin. Niacin that is added to enriched and fortified foods is in its free form and therefore highly bioavailable.

Tryptophan is another food source of niacin because this amino acid—when present in amounts beyond that required for protein synthesis—can be converted to NAD, mainly in the liver. The most commonly used estimate of efficiency for tryptophan conversion to NAD is 1:60 (i.e., 1 mg niacin [NAD] from 60 mg tryptophan). Turkey is an example of a food high in tryptophan; a 3-oz portion of turkey breast meat provides about 180 mg tryptophan, which could be equivalent to 3 mg niacin. However, the efficiency of the conversion of tryptophan to NAD varies considerably in different people.

Selected Food Sources of Vitamin B2

(mg) per
Pan fried beef liver, 3 ounces14.993
Grilled chicken breast, 3 ounces10.364
Canned Marinara sauce, 1 cup10.364
Roasted turkey breast, 3 ounces10.063
Cooked sockeye salmon, 3 ounces8.654
Canned in water tuna, 3 ounces8.654
Pork tenderloin, roasted, 3 ounces6.339
Beef, ground, 90% lean, pan-browned, 3 ounces5.836
Brown rice, cooked, 1 cup5.233
Fortified breakfast cereal5.031
Dry roasted peanuts, 1 ounce4.226
White enriched rice, cooked, 1 cup2.314
Baked russet potato (russet), 1 medium2.314
Dry roasted sunflower seeds, 1 ounce2.013
Whole wheat bread, 1 slice1.48
Dry roasted pumpkin seeds, 1 ounce1.38
Soymilk, unfortified, 1 cup1.38
White enriched bread, 1 slice1.38
Lentils, boiled and drained, ½ cup1.06
Bulgur, cooked, 1 cup0.96
Banana, 1 medium0.85
Cooked edamame, ½ cup0.74
Raisins, ½ cup0.64
Cherry tomatoes, ½ cup0.53
Cooked broccoli, ½ cup0.43
Dry roasted cashews, 1 ounce0.43
Plain yogurt, 1 cup0.32
Apple, 1 medium0.21
Canned chickpeas, drained, 1 cup0.21
Milk, 1 cup0.21
Boiled spinach, ½ cup0.21
Firm tofu, raw, ½ cup0.21
Chopped onions, ½ cup0.11
Egg, large00
  • *DV = Daily Value.
  • The DV for niacin is used as the basis for the values the table above is 16 mg for adults and children age 4 and older.
  • Foods providing 20% or more of the DV are considered to be high sources of a nutrient, but foods providing lower percentages of the DV also contribute to a healthful diet.


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